TNF-ALPHA AND IL-1-BETA UP-REGULATE NITRIC OXIDE-DEPENDENT CILIARY MOTILITY IN BOVINE AIRWAY EPITHELIUM

被引:81
作者
JAIN, B
RUBINSTEIN, I
ROBBINS, RA
SISSON, JH
机构
[1] UNIV NEBRASKA, MED CTR, DEPT MED, PULM & CRIT CARE MED SECT, OMAHA, NE 68198 USA
[2] UNIV NEBRASKA, MED CTR, DEPT PHYSIOL & BIOPHYS, OMAHA, NE 68198 USA
[3] OMAHA VET AFFAIRS MED CTR, RES SERV, OMAHA, NE 68198 USA
关键词
CYTOKINE; MACROPHAGE; MUCOCILIARY CLEARANCE; INFLAMMATION;
D O I
10.1152/ajplung.1995.268.6.L911
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Airway epithelial cells can be modulated by cytokines such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta that are released from inflammatory cells. Since ciliary motility is an important host defense function of airway epithelium, we hypothesized that cytokines, released from lung macrophages, upregulate ciliary motility. To test this hypothesis, ciliary beat frequency (CBF) was measured by video microscopy in cultured ciliated bovine bronchial epithelial cells (BBECs) incubated for 24 h with bovine alveolar macrophage-conditioned medium (AM-CM). Exposure to AM-CM resulted in a delayed (greater than or equal to 2 h) increase in CBF that was maximal after 24 h exposure (13.70 +/- 0.43 for AM-CM vs. 9.44 +/- 0.24 Hz for medium; P < 0.0001) and which was largely blocked by either anti-TNF-alpha or anti-IL-1 beta antibodies. rTNF-alpha or rIL-1 beta similarly increased CBF, which could be blocked by preincubation with either anti-rTNF-alpha or anti-rIL-1 beta antibodies. Preincubation of BBECs with actinomycin D or dexamethasone also blocked rTNF-alpha- and rIL-1 beta-induced cilia stimulation, suggesting that new protein synthesis is required for cytokine-induced upregulation of CBF. Since NO is known to upregulate ciliary motility and cytokines can induce NO synthase (NOS), we hypothesized that TNF-alpha and IL-1 beta increase CBF by inducing NOS in BBECs. The cilia stimulatory effects of TNF-alpha or IL-1 beta were inhibited by N-G-monomethyl-L-arginine, a competitive NOS inhibitor, and restored by the addition of either L-arginine, an NOS substrate, or sodium nitroprusside, an NO donor. These studies show that TNF-alpha or IL-1 beta can upregulate ciliary motility presumably by releasing NO via induction of NOS and suggest a mechanism by which inflammation increases ciliary motility.
引用
收藏
页码:L911 / L917
页数:7
相关论文
共 29 条
[1]   NITRIC-OXIDE AND LUNG-DISEASE [J].
BARNES, PJ ;
BELVISI, MG .
THORAX, 1993, 48 (10) :1034-1043
[2]  
BELENKY SN, 1993, J LAB CLIN MED, V122, P388
[3]  
DOI T, 1993, INFECT IMMUN, V61, P1980, DOI 10.1128/IAI.61.5.1980-1989.1993
[4]  
GREENBERG S, 1993, CLIN RES, V41, pA768
[5]   INDUCTION OF NITRIC-OXIDE SYNTHASE IN ASTHMA [J].
HAMID, Q ;
SPRINGALL, DR ;
RIVEROSMORENO, V ;
CHANEZ, P ;
HOWARTH, P ;
REDINGTON, A ;
BOUSQUET, J ;
GODARD, P ;
HOLGATE, S ;
POLAK, JM .
LANCET, 1993, 342 (8886-7) :1510-1513
[6]  
HUNNINGHAKE GW, 1984, AM REV RESPIR DIS, V129, P569
[7]   MODULATION OF AIRWAY EPITHELIAL-CELL CILIARY BEAT FREQUENCY BY NITRIC-OXIDE [J].
JAIN, B ;
RUBINSTEIN, I ;
ROBBINS, RA ;
LEISE, KL ;
SISSON, JH .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 191 (01) :83-88
[8]  
JORENS PG, 1993, EUR RESPIR J, V6, P258
[9]  
KHAN AR, 1986, EUR J PHARMACOL, V130, P91
[10]   INCREASED NITRIC-OXIDE IN EXHALED AIR OF ASTHMATIC-PATIENTS [J].
KHARITONOV, SA ;
YATES, D ;
ROBBINS, RA ;
LOGANSINCLAIR, R ;
SHINEBOURNE, EA ;
BARNES, PJ .
LANCET, 1994, 343 (8890) :133-135