A CONTINUOUS MULTISTAGE ROLLER REACTOR FOR ANIMAL-CELL CULTURE .1. PATTERNS OF GROWTH, PRODUCTION AND CATABOLISM OF A MURINE HYBRIDOMA

被引：3

作者：

BORTH, N

STEINDL, F

WEIGANG, F

REITER, M

KATINGER, H

机构：

[1] Institute of Applied Microbiology, Vienna, A-1190

来源：

CYTOTECHNOLOGY | 1990年 / 3卷 / 03期

关键词：

catabolic flux rates; continuous hybridoma fermentation; kinetics of antibody production; Tubular Liquid Film Reactor;

D O I：

10.1007/BF00365489

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high cell densities of up to 107 cells/ml were achieved due to cell sedimentation. The model character of the reactor was taken to determine critical values of substrate concentrations that influence production rates and result in an adaptation of metabolism. © 1990 Kluwer Academic Publishers.

引用

页码：253 / 258

页数：6

共 12 条

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