ELISA FOR OXYTOCIN - HIGHLY SENSITIVE TESTS AND APPLICATION FOR THE TITRATION OF AN OXYTOCIN-LIKE SUBSTANCE IN THE LEECH ERPOBDELLA-OCTOCULATA

被引：33

作者：

SALZET, M

WATTEZ, C

SLOMIANNY, MC

LEU, B

SIEGERT, KJ

机构：

[1] Laboratoire d'Endocrinologie des Invertébrés, URA CNRS 148, Université des Sciences et Techniques de Lille

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1992年 / 102卷 / 03期

关键词：

D O I：

10.1016/0742-8413(92)90147-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Three different types of ELISA for oxytocin (OT) were developed: direct, inhibiting and competitive. The amounts of OT which could be detected varied with the type of test: 10 to 100 pmol in direct and inhibiting ELISA, 50 fmol to 10 pmol in competitive ELISA. 2. A comparison between the values of percentage of binding obtained with radioimmunoassay (RIA) and in competitive ELISA showed that this latter assay is as sensitive as the RIA but it offers the advantages of being cheaper and more reproducible. 3. The development of these sensitive ELISA provided a means to quantify OT-like molecules. Their usefulness has been first applied to the central nervous system of the leech, Erpobdella octoculata.

引用

页码：483 / 487

页数：5

共 18 条

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