CAPSULATED CELLS OF AEROMONAS-SALMONICIDA GROWN IN-VITRO HAVE DIFFERENT FUNCTIONAL-PROPERTIES THAN CAPSULATED CELLS GROWN IN-VIVO

被引:18
作者
GARDUNO, RA
KAY, WW
机构
[1] UNIV VICTORIA, DEPT BIOCHEM & MICROBIOL, VICTORIA, BC V8W 3P6, CANADA
[2] UNIV VICTORIA, CANADIAN BACTERIAL DIS NETWORK, VICTORIA, BC V8W 3P6, CANADA
关键词
AEROMONAS SALMONICIDA; BACTERIAL CAPSULES; MIMICRY OF IN VIVO CONDITIONS; FURUNCULOSIS VACCINES;
D O I
10.1139/m95-130
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When grown in vivo in the peritoneal cavity of rainbow trout, Aeromonas salmonicida produces a clearly defined capsule with virulence-related functions. Aeromonas salmonicida grown in vitro in a glucose-rich medium (GRM) has also been reported to produce capsular material. Because in vitro mimicry of in vivo induced traits is highly desirable in vaccine design, the extent to which growth in GRM mimicked in vivo growth was examined. Antibodies specific to in vivo grown cells partially labeled the surface of GRM-grown cells, as well as two distinct proteins (81 700 and 41 000 M(r)) in immunoblots of mutants with S-layer or lipopolysaccharide defects. GRM-grown strains showed an increased sensitivity to trout serum in contradistinction to the complete serum resistance of in vivo grown cells; as well, GRM-grown cells were more adherent to trout macrophages. Thus in spite of possessing some surface antigens normally expressed in vivo, cells grown on solid GRM did not possess all functional properties of in vivo gown cells.
引用
收藏
页码:941 / 945
页数:5
相关论文
共 14 条
[1]   EFFECTS OF NUTRIENTS ON EXOPOLYSACCHARIDE PRODUCTION AND SURFACE-PROPERTIES OF AEROMONAS-SALMONICIDA [J].
BONET, R ;
SIMONPUJOL, MD ;
CONGREGADO, F .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (08) :2437-2441
[2]   THE PHYSIOLOGICAL-ACTIVITY OF BACTERIA ATTACHED TO SOLID-SURFACES [J].
FLETCHER, M .
ADVANCES IN MICROBIAL PHYSIOLOGY, 1991, 32 :53-85
[3]  
GARDUNO RA, 1992, INFECT IMMUN, V60, P4612
[4]  
GARDUNO RA, 1993, INFECT IMMUN, V61, P3854
[5]  
GARROTE A, 1992, FEMS MICROBIOL LETT, V95, P127, DOI 10.1111/j.1574-6968.1992.tb05354.x
[6]   EFFECTS OF INVITRO AND INVIVO GROWTH-CONDITIONS ON EXPRESSION OF TYPE-8 CAPSULAR POLYSACCHARIDE BY STAPHYLOCOCCUS-AUREUS [J].
LEE, JC ;
TAKEDA, S ;
LIVOLSI, PJ ;
PAOLETTI, LC .
INFECTION AND IMMUNITY, 1993, 61 (05) :1853-1858
[7]   A NEW TEST BASED ON SALTING OUT TO MEASURE RELATIVE SURFACE HYDROPHOBICITY OF BACTERIAL-CELLS [J].
LINDAHL, M ;
FARIS, A ;
WADSTROM, T ;
HJERTEN, S .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 677 (3-4) :471-476
[8]   INVITRO SIMULATION OF INVIVO CONDITIONS - PHYSICAL STATE OF THE CULTURE-MEDIUM [J].
LORIAN, V .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (11) :2403-2406
[9]   ROLE OF SURFACE COMPONENTS IN SERUM RESISTANCE OF VIRULENT AEROMONAS-SALMONICIDA [J].
MUNN, CB ;
ISHIGURO, EE ;
KAY, WW ;
TRUST, TJ .
INFECTION AND IMMUNITY, 1982, 36 (03) :1069-1075
[10]  
PHIPPS BM, 1988, J BIOL CHEM, V263, P9298