FRACTIONATION OF HISTONES OF GROUP F2(B) FROM CALF THYMUS

被引:19
作者
JOHNS, EW
机构
[1] Chester Beatty Research Institute Institute of Cancer Research:, London, Royal Cancer Hospital Fulham Road
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1968年 / 4卷 / 04期
关键词
D O I
10.1111/j.1432-1033.1968.tb00231.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The histones of group F2(b) have been fractionated by acetone precipitation and by column chromatography on carboxymethylcellulose at pH 9. The acetone precipitation method enables F2(b), prepared on a large scale by extraction methods, to be further purified in bulk, the resulting product having 84% N‐terminal proline. The column chromatography on carboxymethylcellulose at pH 9 has given 5 main fractions, two of which contain over 25% acidic amino acids (or their amides) and over 24% basic amino acids. Copyright © 1968, Wiley Blackwell. All rights reserved
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页码:437 / &
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