A RADIATION-REDUCED HYBRID CELL-LINE CONTAINING 5 MB/17 CM OF HUMAN DNA FROM 9Q34

被引：13

作者：

HENSKE, EP

OZELIUS, L

ANDERSON, MA

KWIATKOWSKI, DJ

机构：

[1] HARVARD UNIV, SCH MED, DEPT GENET, BOSTON, MA 02115 USA

[2] MASSACHUSETTS GEN HOSP, MOLEC NEUROGENET LAB, BOSTON, MA 02114 USA

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90165-O

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Disease gene loci for tuberous sclerosis (TSC1), idiopathic torsion dystonia (DYT1), and nail-patella syndrome (NPS1) have been mapped by genetic linkage analysis to human chromosome 9q band 34. To create a resource for physical mapping and manipulation of this region of the genome, we have created a radiation-reduced hybrid cell line containing DNA from human 9q34 as its only human component. This cell line, E6B, has been characterized by Southern blot and PCR analysis using a panel of 9q markers and fluorescent in situ hybridization. We estimate that it contains 5 Mb of human DNA, equal to 17 cM of genetic distance, extending from AK1 to ABO on 9q34. © 1992.

引用

页码：841 / 844

页数：4

共 17 条

[1] BUETOW KH, 1991, AM J HUM GENET, V48, P911
[2] REGIONAL ASSIGNMENTS OF THE LOCI AK3, ACONS, AND ASS ON HUMAN CHROMOSOME-9
CARRITT, B
POVEY, S
[J]. CYTOGENETICS AND CELL GENETICS, 1979, 23 (03): : 171 - 181
[3] BASE SEQUENCE STUDIES OF 300 NUCLEOTIDE RENATURED REPEATED HUMAN DNA CLONES
DEININGER, PL
JOLLY, DJ
RUBIN, CM
FRIEDMANN, T
SCHMID, CW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1981, 151 (01) : 17 - 33
[4] FRYER AE, 1987, LANCET, V1, P659
[5] GOSS SJ, 1977, J CELL SCI, V25, P17
[6] HARRIS RM, 1992, IN PRESS CYTOGENET C
[7] EXPRESSION OF THE HUMAN ARGININOSUCCINATE SYNTHETASE GENE IN HAMSTER TRANSFERENTS
HUDSON, LD
ERBE, RW
JACOBY, LB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (07): : 4234 - 4238
[8] JONES C, 1984, CYTOGENET CELL GENET, V37, P499
[9] CONSTRUCTION OF A GT POLYMORPHISM MAP OF HUMAN 9Q
KWIATKOWSKI, DJ
HENSKE, EP
WEIMER, K
OZELIUS, L
GUSELLA, JF
HAINES, J
[J]. GENOMICS, 1992, 12 (02) : 229 - 240
[10] KWIATKOWSKI DJ, 1991, AM J HUM GENET, V49, P366

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