AN IMPROVED METHOD FOR MONITORING ACTIVE OXYGEN IN BACTERIA-TREATED SUSPENSION CELLS USING LUMINOL-DEPENDENT CHEMILUMINESCENCE

被引：60

作者：

GLAZENER, JA

ORLANDI, EW

HARMON, GL

BAKER, CJ

机构：

[1] Microbiology and Plant Pathology Laboratory, United States Department of Agriculture, ARS, Beltsville

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 1991年 / 39卷 / 02期

关键词：

D O I：

10.1016/0885-5765(91)90023-B

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Modification of luminol-dependent chemiluminescence (LDC) assays resulted in a reliable and highly reproducible method for the measurement of active oxygen levels in bacteria-treated soybean suspension cells. Optimization of the LDC assay for use at pH 7·0 required the addition of horseradish peroxidase (HRP) and the continuous monitoring of chemiluminescence to detect accurate maximum peak heights. With this improved technique, we were able to detect changes in active oxygen in soybean cell suspension cultures inoculated with Pseudomonas syringae pv. glycinea races 4 and 6. During the first hour after inoculation, increased active oxygen was observed in cells inoculated with either race. A second peak occurred after 3-4 h in suspension cell cultures inoculated with race 6, which causes a hypersensitive reaction in soybean leaves. © 1991.

引用

页码：123 / 133

页数：11

共 17 条

[1] CONSEQUENCE OF O2(-) GENERATION DURING A BACTERIALLY INDUCED HYPERSENSITIVE REACTION IN TOBACCO - DETERIORATION OF MEMBRANE-LIPIDS [J].