ENGINEERING MULTIPLE PROPERTIES OF A PROTEIN BY COMBINATORIAL MUTAGENESIS

被引：64

作者：

SANDBERG, WS

TERWILLIGER, TC

机构：

[1] UNIV CHICAGO,DEPT BIOCHEM & MOLEC BIOL,CHICAGO,IL 60637

[2] LOS ALAMOS NATL LAB,DIV LIFE SCI,LOS ALAMOS,NM 87545

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 18期

关键词：

PROTEIN ENGINEERING; ADDITIVITY; STABILITY; DNA BINDING PROTEIN;

D O I：

10.1073/pnas.90.18.8367

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method for simultaneously engineering multiple properties of a protein, based on the observed additivity of effects of individual mutations, is presented. We show that, for the gene V protein of bacteriophage f1, effects of double mutations on both protein stability and DNA binding affinity are approximately equal to the sums of the effects of the constituent single mutations. This additivity of effects implies that it is possible to deliberately construct mutant proteins optimized for multiple properties by combination of appropriate single mutations chosen from a characterized library.

引用

页码：8367 / 8371

页数：5

共 45 条

[1] CONTRIBUTIONS OF HYDROGEN-BONDS OF THR-157 TO THE THERMODYNAMIC STABILITY OF PHAGE-T4 LYSOZYME
ALBER, T
SUN, DP
WILSON, K
WOZNIAK, JA
COOK, SP
MATTHEWS, BW
[J]. NATURE, 1987, 330 (6143) : 41 - 46
[2] FLUORESCENCE STUDIES OF THE COMPLEX-FORMATION BETWEEN THE GENE-5 PROTEIN OF BACTERIOPHAGE-M13 AND POLYNUCLEOTIDES
ALMA, NCM
HARMSEN, BJM
DEJONG, EAM
VANDERVEN, J
HILBERS, CW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1983, 163 (01) : 47 - 62
[3] [Anonymous], 1985, ENZYME STRUCTURE MEC
[4] REFINED STRUCTURE OF THE GENE-5 DNA-BINDING PROTEIN FROM BACTERIOPHAGE-FD
BRAYER, GD
MCPHERSON, A
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1983, 169 (02) : 565 - 596
[5] BRYAN P N, 1986, Proteins Structure Function and Genetics, V1, P326
[6] SPECIFICITY OF THE BINDING OF BACTERIOPHAGE-M13 ENCODED GENE-5 PROTEIN TO DNA AND RNA STUDIED BY MEANS OF FLUORESCENCE TITRATIONS
BULSINK, H
HARMSEN, BJM
HILBERS, CW
[J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1985, 3 (02) : 227 - 247
[7] ENGINEERING SUBTILISIN BPN' FOR SITE-SPECIFIC PROTEOLYSIS
CARTER, P
NILSSON, B
BURNIER, JP
BURDICK, D
WELLS, JA
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1989, 6 (03): : 240 - 248
[8] ENGINEERING ENZYME SPECIFICITY BY SUBSTRATE-ASSISTED CATALYSIS
CARTER, P
WELLS, JA
[J]. SCIENCE, 1987, 237 (4813) : 394 - 399
[9] DISSECTING THE CATALYTIC TRIAD OF A SERINE PROTEASE
CARTER, P
WELLS, JA
[J]. NATURE, 1988, 332 (6164) : 564 - 568
[10] A MUTANT T4 LYSOZYME (VAL 131-]ALA) DESIGNED TO INCREASE THERMOSTABILITY BY THE REDUCTION OF STRAIN WITHIN AN ALPHA-HELIX
DAOPIN, S
BAASE, WA
MATTHEWS, BW
[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1990, 7 (02) : 198 - 204

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