CERIUM(IV)-OLIGODNA HYBRID AS HIGHLY SELECTIVE ARTIFICIAL NUCLEASE

被引：37

作者：

KOMIYAMA, M

SHIIBA, T

TAKAHASHI, Y

TAKEDA, N

MATSUMURA, K

KODAMA, T

机构：

[1] UNIV TOKYO,FAC ENGN,DEPT IND CHEM,TOKYO 113,JAPAN

[2] UNIV TSUKUBA,INST MAT SCI,TSUKUBA,IBARAKI 305,JAPAN

来源：

SUPRAMOLECULAR CHEMISTRY | 1994年 / 4卷 / 01期

关键词：

D O I：

10.1080/10610279408029859

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cerium(IV) ion is attached to the 5'-end of a 19-mer DNA by use of iminodiacetate Ligand. At pH 7.2 and 30 degrees C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3'-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidylyl(3'-5')thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.

引用

页码：31 / 34

页数：4

共 16 条

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Adams R. L. P., 1986, BIOCH NUCLEIC ACIDS

[2]

[Anonymous], 1971, SPECIAL PUBLICATION, V25

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