SODIUM-BUTYRATE INHIBITS EXPRESSION OF UROKINASE AND ITS RECEPTOR MESSENGER-RNAS AT BOTH TRANSCRIPTION AND POST-TRANSCRIPTION LEVELS IN COLON-CANCER CELLS

被引：24

作者：

DANG, JJ ^{[1
]}

WANG, Y ^{[1
]}

DOE, WF ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DIV CLIN SCI,CANBERRA,ACT 2601,AUSTRALIA

来源：

FEBS LETTERS | 1995年 / 359卷 / 2-3期

关键词：

BUTYRATE; UROKINASE; UROKINASE RECEPTOR; TUMOR NECROSIS FACTOR;

D O I：

10.1016/0014-5793(95)00029-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of butyrate on the modulation of urokinase plasminogen activator (uPA) and its receptor (uPAR) mRNAs were studied, While both mRNA levels were increased after stimulation by tumor necrosis factor alpha (TNF alpha), phorbol ester (PMA) and cycloheximide, they were inhibited by butyrate at 2.5 to 25 mM. Nuclear run-on transcription assays indicated that uPA mRNA was modulated by butyrate at the transcriptional level but the uPAR gene was regulated at both transcriptional and post-transcriptional levels in the presence or absence of TNF alpha. In the presence of PMA, however, butyrate acts at the posttranscriptional level on both genes.

引用

页码：147 / 150

页数：4

共 28 条

[1]

Ausubel FM, 1987, CURRENT PROTOCOLS MO

[2]

BAKER MS, 1990, CANCER RES, V50, P4676

[3] THE HUMAN UBIQUITIN GENE FAMILY - STRUCTURE OF A GENE AND PSEUDOGENES FROM THE UB-B SUBFAMILY [J].

BAKER, RT ;

BOARD, PG .

NUCLEIC ACIDS RESEARCH, 1987, 15 (02) :443-463

[4] REGULATION OF TUMOR NECROSIS FACTOR GENE-EXPRESSION IN COLORECTAL ADENOCARCINOMA - INVIVO ANALYSIS BY INSITU HYBRIDIZATION [J].

BEISSERT, S ;

BERGHOLZ, M ;

WAASE, I ;

LEPSIEN, G ;

SCHAUER, A ;

PFIZENMAIER, K ;

KRONKE, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (13) :5064-5068

[5] UROKINASE AND UROKINASE RECEPTOR - A PARACRINE AUTOCRINE SYSTEM REGULATING CELL-MIGRATION AND INVASIVENESS [J].

BLASI, F .

BIOESSAYS, 1993, 15 (02) :105-111

[6]

BRATTAIN MG, 1981, CANCER RES, V41, P1751

[7] UROKINASE-TYPE PLASMINOGEN-ACTIVATOR AND ITS RECEPTOR - NEW TARGETS FOR ANTIMETASTATIC THERAPY [J].

FAZIOLI, F ;

BLASI, F .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1994, 15 (01) :25-29

[8] IDENTIFICATION OF SEQUENCE ELEMENTS IN MOUSE CALBINDIN-D(28K) GENE THAT CONFER 1,25-DIHYDROXYVITAMIN-D(3)-INDUCIBLE AND BUTYRATE-INDUCIBLE RESPONSES [J].

GILL, RK ;

CHRISTAKOS, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :2984-2988

[9] SODIUM-BUTYRATE INDUCES APOPTOSIS IN HUMAN COLONIC TUMOR-CELL LINES IN A P53-INDEPENDENT PATHWAY - IMPLICATIONS FOR THE POSSIBLE ROLE OF DIETARY FIBER IN THE PREVENTION OF LARGE-BOWEL CANCER [J].

HAGUE, A ;

MANNING, AM ;

HANLON, KA ;

HUSCHTSCHA, LI ;

HART, D ;

PARASKEVA, C .

INTERNATIONAL JOURNAL OF CANCER, 1993, 55 (03) :498-505

[10]

HEERDT BG, 1994, CANCER RES, V54, P3288

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