HIGHLY PURIFIED PHOTOSYNTHETIC REACTION-CENTER (PSCA/CYTOCHROME C(551))(2) COMPLEX OF THE GREEN SULFUR BACTERIUM CHLOROBIUM-LIMICOLA

被引：31

作者：

OHOKA, H ^{[1
]}

KAKUTANI, S ^{[1
]}

KAMEI, S ^{[1
]}

MATSUBARA, H ^{[1
]}

IWAKI, M ^{[1
]}

ITOH, S ^{[1
]}

机构：

[1] NATL INST BASIC BIOL,OKAZAKI,AICHI 444,JAPAN

来源：

BIOCHEMISTRY | 1995年 / 34卷 / 40期

关键词：

D O I：

10.1021/bi00040a021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photosynthetic reaction center (RC) complex that forms a homodimer of core and cytochrome c subunits was isolated from Chlorobium limicola f. thiosulfatophilum, strain Larsen. The complex showed only two subunit bands at 68 (PscA core) and 21 kDa (cytochrome c(551)) on SDS-PAGE analysis, indicating the complete deletion of the light-harvesting bacteriochlorophyll a (BChl a) protein as well as the iron-sulfur protein. It contained 27 +/- 3 molecules of BChl a, 7 +/- 1 Chl-670, 3 +/- 1 carotenoids, and 1.6 +/- 0.1 c-type hemes per the primary electron donor P840. The complex showed a light-induced charge separation and recombination between P840 and the acceptor Chl-670 at 77 K as follows: P840*Chl-670 --> P840(+)Chl-670(-) --> P840(T)Chl-670(-) --> P840 Chl-670. Pigment compositions and their function in the (PscA/cytochrome c(551))(2) complex were studied by absorption, circular dichroism, and fluorescence spectroscopy.

引用

页码：13091 / 13097

页数：7

共 36 条

[1] ORIGIN AND EARLY EVOLUTION OF PHOTOSYNTHESIS [J].