TRANSMISSION-BLOCKING ANTIBODIES RECOGNIZE MICROFILARIAL CHITINASE IN BRUGIAN LYMPHATIC FILARIASIS

被引：102

作者：

FUHRMAN, JA

LANE, WS

SMITH, RF

PIESSENS, WF

PERLER, FB

机构：

[1] NEW ENGLAND BIOLABS INC,BEVERLY,MA 01915

[2] HARVARD UNIV,MICROCHEM FACIL,CAMBRIDGE,MA 02138

[3] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,MOLEC BIOL COMP RES RESOURCE,BOSTON,MA 02115

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 05期

关键词：

BRUGIA-MALAYI; PARASITIC NEMATODES; PCR;

D O I：

10.1073/pnas.89.5.1548

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. The monoclonal antibody MF1, which mediates clearance of peripheral microfilaremia in a gerbil infection model, recognizes two stage-specific proteins, p70 and p75, in B. malayi microfilariae. cDNA coding for the MF1 antigen was sequenced, and the predicted protein sequence shows significant similarities to chitinases from bacteria and yeast. When microfilarial extracts and purified preparations of the MF1 antigen were tested for chitinase activity, strong bands of chitin-degrading activity comigrated in SDS/PAGE with p70 and p75 and showed a reduction-dependent mobility shift characteristic of the MF1 antigen. Thus, the MF1 antigen is microfilarial chitinase, which may function to degrade chitin-containing structures in the microfilaria or in its mosquito vector during parasite development and transmission.

引用

页码：1548 / 1552

页数：5

共 25 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].