IMMUNOGOLD LABELING OF ARABINOXYLANS IN THE PLANT-CELL WALLS OF NORMAL AND BM3 MUTANT MAIZE

被引：10

作者：

BARRY, P

PRENSIER, G

GRENET, E

机构：

[1] INRA,RECH NUTR HERBIVORES STN,UNITE INGEST,F-63122 CEYRAT,FRANCE

[2] UNIV CLERMONT FERRAND,MICROBIOL LAB,F-63177 CLERMONT FERRAND,FRANCE

来源：

BIOLOGY OF THE CELL | 1991年 / 71卷 / 03期

关键词：

PLANT CELL WALL; XYLANS; IMMUNOCYTOCHEMISTRY; MAIZE;

D O I：

10.1016/0248-4900(91)90275-R

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Polyclonal antibodies directed against alpha, L-1.3-arabinofuranosyl poly-beta,D-1.4-xylopyranosyl (degree of polymerization 130) have been raised from rabbits. The immunogold labelling in transmission electron microscopy (TEM) evidenced the arabinoxylans of the plant cell walls. Comparison between the stems of normal and mutant bm3 maize demonstrated a greater accessibility of arabinoxylans in the walls of the mutant maize. The method, specific and swift, allows us to specify the repartition in the different parts of the stem: sclerenchyma, fibers, parenchyma.

引用

页码：307 / 311

页数：5

共 18 条

[1] LIGNIN CARBOHYDRATE COMPLEXES CONTAINING PHENOLIC-ACIDS ISOLATED FROM THE CULMS OF BAMBOO [J].