PHOTOREACTION OF N560 INTERMEDIATE IN THE PHOTOCYCLE OF BACTERIORHODOPSIN

被引：15

作者：

YAMAMOTO, N ^{[1
]}

NARAMOTO, S ^{[1
]}

OHTANI, H ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,DEPT BIOMOLEC ENGN,4259 NAGATSUTA,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

FEBS LETTERS | 1992年 / 314卷 / 03期

关键词：

BACTERIORHODOPSIN; PURPLE MEMBRANE IN ALKALINE SUSPENSION; SECONDARY PHOTOCYCLE; N560; R660;

D O I：

10.1016/0014-5793(92)81502-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sophisticated measurements were made on the nanosecond time-resolved absorbance change of the purple membrane of Halobacterium halobium under cw background light irradiation (440-800 nm, 11-441 mW/cm2). A red-shifted transient species R660 (K(N), Q) was found in alkaline conditions (pH > 9.3). Background light intensity effect shows that (i) R660 is photochemically formed from N560 intermediate which is accumulated under background light irradiation because of the elongated lifetime in alkaline suspension, and that (ii) the slow decaying M412 is not photochemically formed from N560 but from bR568.

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页码：345 / 347

页数：3

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