EPIDERMAL GROWTH FACTOR-STIMULATED CALCIUM-ION TRANSIENTS IN INDIVIDUAL A431 CELLS - INITIATION KINETICS AND LIGAND CONCENTRATION-DEPENDENCE

被引:29
作者
CHEYETTE, TE
GROSS, DJ
机构
[1] UNIV MASSACHUSETTS,DEPT BIOCHEM,AMHERST,MA 01003
[2] UNIV MASSACHUSETTS,PROGRAM MOLEC & CELLULAR BIOL,AMHERST,MA 01003
来源
CELL REGULATION | 1991年 / 2卷 / 10期
关键词
D O I
10.1091/mbc.2.10.827
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The A431 epidermoid carcinoma cell line responds to epidermal growth factor (EGF) stimulation with a number of rapid changes, including alterations in free cytosolic calcium ion concentration ([Ca2+]i). At the single cell level, these changes in [Ca2+]i are known to proceed after a clear lag phase subsequent to EGF stimulus (Gonzalez et al, 1988). The present study explores the dependence on EGF concentration of this early [Ca2+]i signal. High levels of EGF (9.0-4.3 nM) produce a [Ca2+]i spike followed by an elevation of [Ca2+]i above basal levels. The time of initiation of the spike varies from 5 to 9 s at the high dose and from 8 to 32 s at the low dose in cells that respond. A lower level of EGF (1.5 nM) produces [Ca2+]i oscillations with no prolonged elevation over basal [Ca2+]i. The initiation of response at this [EGF] ranges from 20 to 410 s. Intermediate stimulus levels generate [Ca2+]i responses that are kinetic admixtures of these limiting responses. A simple model based on the enzymatically amplified signal cascade from ligand binding through Ca2+ release or influx is examined. The model predicts a prolonged lag phase followed by a rapid increase in the [Ca2+]i signal that compares favorably with the data reported here.
引用
收藏
页码:827 / 840
页数:14
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