COMPARATIVE EFFECTS OF GALANIN ON ISOLATED SMOOTH-MUSCLE CELLS FROM ILEUM IN 5 MAMMALIAN-SPECIES

被引：55

作者：

BOTELLA, A ^{[1
]}

DELVAUX, M ^{[1
]}

FREXINOS, J ^{[1
]}

BUENO, L ^{[1
]}

机构：

[1] CHU RANGUEIL,DIGEST MOTIL LAB,F-31054 TOULOUSE,FRANCE

来源：

LIFE SCIENCES | 1992年 / 50卷 / 17期

关键词：

D O I：

10.1016/0024-3205(92)90325-J

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Effect of galanin and CCK8 were studied on isolated smooth muscle cells obtained from pig, guinea-pig, rat, rabbit and dog ileum circular muscle layer. Galanin as well as CCK8 induced a concentration-dependent contraction of pig, rat, rabbit and guinea-pig ileum smooth muscle cells. Maximal contraction ranged between 23.7 +/- 1.9% and 26.1 +/- 3.1% decrease in cell length from control in the presence of both peptides. This maximal contraction was obtained at 1 nM galanin in pig, rat, rabbit, 1 nM CCK8 in rat, rabbit, guinea-pig, at 10 nM galanin in guinea-pig and 10 nM CCK8 in pig. Concentrations of galanin inducing a half maximal contraction (EC50) ranged between 8 pM and 80 pM in these species. In dog, CCK8 induced a concentration-dependent contraction of ileum smooth muscle cells, with a maximal contraction (24.5 +/- 2.3%) at 1nM and an EC50 of 50 pM while galanin inhibited cell contraction induced by CCK8. The CCK-induced contraction was abolished at 10 nM galanin and 10 nM VIP. Concentrations of galanin and VIP inducing a half-maximal relaxation of contracted cells were 2 pM and 3 pM respectively. It is concluded that galanin may induce cell contraction of pig, guinea-pig, rat and rabbit ileum circular muscle layer and cell relaxation of dog ileum by a direct myogenic effect.

引用

页码：1253 / 1261

页数：9

共 36 条

[1] DISTINCT ACTIVATION OF NA+-H+ EXCHANGE BY GASTRIN AND CCK PEPTIDE IN ACINI FROM GUINEA-PIG [J].