THE ADA PROTEIN IS A CLASS-I TRANSCRIPTION FACTOR OF ESCHERICHIA-COLI

被引：25

作者：

SAKUMI, K

IGARASHI, K

SEKIGUCHI, M

ISHIHAMA, A

机构：

[1] NATL INST GENET,DEPT MOLEC GENET,MISHIMA,SHIZUOKA 411,JAPAN

[2] KYUSHU UNIV,DEPT BIOCHEM,MED INST BIOREGULAT,FUKUOKA 812,JAPAN

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 08期

关键词：

D O I：

10.1128/JB.175.8.2455-2457.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The methylated Ada protein of Escherichia coli, a regulatory protein for the adaptive response, binds to a target DNA from positions -62 to -31 upstream of the ada gene and facilitates the binding of RNA polymerase to the promoter. Mutant RNA polymerases consisting of C-terminal-deleted alpha subunits are virtually inactive in response to activation by the Ada protein. Thus, we conclude that the Ada protein is a class I transcription factor which requires the C-terminal region of the RNA polymerase alpha subunit for transcription activation.

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页码：2455 / 2457

页数：3

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