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CLONING AND SEQUENCE-ANALYSIS OF MOUSE CDNAS ENCODING PREPROTACHYKININ-A AND PREPROTACHYKININ-B

被引：13
作者：
KAKO, K
MUNEKATA, E
HOSAKA, M
MURAKAMI, K
NAKAYAMA, K
机构：
[1] UNIV TSUKUBA,CTR GENE EXPT,TSUKUBA,IBARAKI 305,JAPAN
[2] UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN
来源：
BIOMEDICAL RESEARCH-TOKYO | 1993年 / 14卷 / 04期
关键词：
D O I：
10.2220/biomedres.14.253
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We describe here the isolation and characterization of cDNAs from mouse brain coding for the precursor of substance P and neurokinin A, preprotachykinin A (PPT-A), and for the precursor of neurokinin B, preprotachykinin B (PPT-B). Two types of mouse PPT-A cDNA encoding beta-PPT-A and gamma-PPT-A were obtained. The latter lacked the coding region corresponding to the exon 4 of the PPT-A gene, as is observed in other species. Mouse beta-PPT-A deduced from the cDNA sequence consisted of 130 amino acids, and showed extensive homology to rat, human, and bovine beta-PPT-As, with 99%, 95%, 92% amino acid identities, respectively. The substance P and neurokinin A sequences were the same among these species. Mouse PPT-B deduced from the cDNA sequence consisted of 116 amino acids, and shows homology to rat and bovine PPT-Bs, with 93% and 78% amino acid identities, respectively; thus the homology of PPT-B among these species is less prominent than that of PPT-A. The neurokinin B sequence was the same among these species.
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页码：253 / 259
页数：7
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