DETECTION OF WHITE-ROT FUNGI BY A NON-TOXIC STAIN

被引：240

作者：

NIKUPAAVOLA, ML ^{[1
]}

RAASKA, L ^{[1
]}

ITAVAARA, M ^{[1
]}

机构：

[1] VALT TEKNILLINEN TUTKIMUSKESKUS, TECH RES CTR FINLAND, FOOD RES LAB, SF-02150 ESPOO, FINLAND

来源：

MYCOLOGICAL RESEARCH | 1990年 / 94卷

关键词：

Enzymatic stain; Identification; Isoenzyme; Laccase; White-rot fungi; Wood-rotting fungi;

D O I：

10.1016/S0953-7562(09)81260-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2′-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry. © 1990, British Mycological Society. All rights reserved.

引用

页码：27 / 31

页数：5

共 18 条

[1] IMPORTANCE OF PHENOL OXIDASE ACTIVITY IN LIGNIN DEGRADATION BY WHITE-ROT FUNGUS SPOROTRICHUM-PULVERULENTUM
ANDER, P
ERIKSSON, KE
[J]. ARCHIVES OF MICROBIOLOGY, 1976, 109 (1-2) : 1 - 8
[2] Bavendamm W., 1928, Z PFLANZENKRANKH, V38, P257
[3] Cartwright K.St.G., 1958, DECAY TIMBER ITS PRE
[4] HARKIN JM, 1973, EXPERIENTIA, V29, P382, DOI 10.1007/BF01926734
[5] HINTIKKA V, 701 I FOR FENN COMM
[6] KAARIK A, 1965, STUDIA FORESTALIA SU, V31
[7] PRODUCTION OF MULTIPLE LIGNINASES BY PHANEROCHAETE-CHRYSOSPORIUM - EFFECT OF SELECTED GROWTH-CONDITIONS AND USE OF A MUTANT STRAIN
KIRK, TK
CROAN, S
TIEN, M
MURTAGH, KE
FARRELL, RL
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (01) : 27 - 32
[8] Kirk TK, 1985, BIOSYNTHESIS BIODEGR, P579
[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[10] LEATHAM GF, 1981, J GEN MICROBIOL, V125, P147

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