DEVELOPMENT OF A ROTAVIRUS PLAQUE-ASSAY USING SEPHADEX G-75

被引：12

作者：

AHA, PM

SABARA, MI

机构：

[1] Praxis Biologics Inc., Rochester

来源：

JOURNAL OF VIROLOGICAL METHODS | 1990年 / 28卷 / 01期

关键词：

Overlay; Plaque assay; Rotavirus;

D O I：

10.1016/0166-0934(90)90084-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, reproducible and easily performed plaque assay is described for use with a variety of rotavirus strains. Plaque formation was induced in MA-104 cells by the use of Sephadex G-75, instead of the traditional agar, and crystalline trypsin in the overlay. Formation of large, discrete, easily read plaques was noted in both human and non-human rotavirus strains. © 1990.

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页码：25 / 32

页数：8

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