A NEW METHOD FOR THE INVITRO SCREENING OF INHIBITORS OF ANGIOTENSIN-CONVERTING ENZYME (ACE), USING THE CHROMOPHORE-LABELED AND FLUOROPHORE-LABELED SUBSTRATE, DANSYLTRIGLYCINE

被引:52
作者
ELBL, G [1 ]
WAGNER, H [1 ]
机构
[1] UNIV MUNICH,INST PHARMACEUT BIOL,KARLSTR 29,W-8000 MUNICH 2,GERMANY
关键词
ANTIHYPERTENSIVE PLANT CONSTITUENTS; SCREENING; INVITRO ASSAY; ANGIOTENSIN-CONVERTING ENZYME INHIBITORS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I
10.1055/s-2006-960050
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In our search for antihypertensive plant constituents, we developed a new in vitro assay for the detection of angiotensin-converting enzyme inhibitors in plant extracts. The chromophore- and fluorophore-labelled tripeptide dansyltriglycine is used as substrate, which is cleaved by the enzyme into dansylglycine and diglycine. The product dansylglycine and unreacted substrate are separated and quantified by reversed phase high performance liquid chromatography with UV detection. This target-directed enzyme assay is very specific, highly sensitive and reproducible, and lends itself very easily to automation.
引用
收藏
页码:137 / 141
页数:5
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