ISOLATION OF PURE POPULATIONS OF EPITHELIAL AND MYOEPITHELIAL CELLS FROM THE NORMAL HUMAN MAMMARY-GLAND USING IMMUNOMAGNETIC SEPARATION WITH DYNABEADS

被引：80

作者：

GOMM, JJ ^{[1
]}

BROWNE, PJ ^{[1
]}

COOPE, RC ^{[1
]}

LIU, QY ^{[1
]}

BULUWELA, L ^{[1
]}

COOMBES, RC ^{[1
]}

机构：

[1] UNIV LONDON,CHARING CROSS & WESTMINSTER MED SCH,DEPT BIOCHEM,LONDON W6 8RP,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1196

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies to epithelial membrane antigen and common acute lymphoblastic leukemia antigen were bound to second antibody-coated magnetic microspheres. These specific antibody/bead complexes were then used to directly isolate purified epithelial and myoepithelial cells from normal breast organoid cell preparations by magnetic separation. Near homogeneous cell populations were selected with yields of 10-20 X 10(6) epithelial and myoepithelial cells per organoid preparation. Repeated purification steps allowed almost complete depletion of myoepithelial cells for RNA studies. Tissue culture of separated cell populations in appropriate defined media further ensured purity of cell type and was unimpeded by Dynabead attachment. (C) 1995 Academic Press, Inc.

引用

页码：91 / 99

页数：9

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