A VASOACTIVE PEPTIDE, ENDOTHELIN-3, IS PRODUCED BY AND SPECIFICALLY BINDS TO PRIMARY ASTROCYTES

被引：124

作者：

EHRENREICH, H ^{[1
]}

KEHRL, JH ^{[1
]}

ANDERSON, RW ^{[1
]}

RIECKMANN, P ^{[1
]}

VITKOVIC, L ^{[1
]}

COLIGAN, JE ^{[1
]}

FAUCI, AS ^{[1
]}

机构：

[1] NIAID, BIOL RESOURCES BRANCH, BETHESDA, MD 20892 USA

来源：

BRAIN RESEARCH | 1991年 / 538卷 / 01期

关键词：

ASTROCYTE; GLIAL CELL; BRAIN; ENDOTHELIN-3; VASOACTIVE PEPTIDE; ENDOTHELIN-RECEPTOR;

D O I：

10.1016/0006-8993(91)90375-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Primary rat astrocytes were found by immunohistochemistry to display positive staining for endothelin-3, located predominantly in the perinuclear area. The ability of these cells to produce and release endothelin-3 was confirmed by a combination of reverse-phase HPLC and radioimmunoassay, specific for endothelin-3, which demonstrated immunoreactive peptide in cellular extracts and astrocyte-conditioned medium. In addition, astrocytes were shown to possess a single class of binding sites for endothelin with comparable high affinity for endothelin-1, -2 and -3. These results suggest that astrocytes, by virtue of their ability to produce and secrete endothelin-3, serve as a potential extravascular source of intracerebral vasoregulation capable of influencing regional cerebral blood flow.

引用

页码：54 / 58

页数：5

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