EXACT CLEAVAGE SITE OF ALZHEIMER AMYLOID PRECURSOR IN NEURONAL PC-12 CELLS

被引：202

作者：

ANDERSON, JP

ESCH, FS

KEIM, PS

SAMBAMURTI, K

LIEBERBURG, I

ROBAKIS, NK

机构：

[1] MT SINAI MED CTR,DEPT PSYCHIAT,BOX 1229,1 GUSTAVE LEVY PL,NEW YORK,NY 10029

[2] ATHENA NEUROSCI INC,S SAN FRANCISCO,CA

[3] MT SINAI MED CTR,FISHBERG RES CTR NEUROBIOL,NEW YORK,NY 10029

来源：

NEUROSCIENCE LETTERS | 1991年 / 128卷 / 01期

关键词：

ALZHEIMER DISEASE; ALZHEIMER AMYLOID PRECURSOR PROTEIN; SECRETORY PROTEASE; PC-12; CELL;

D O I：

10.1016/0304-3940(91)90775-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys16-Leu17 bond in the beta-amyloid peptide, followed by partial removal of Lys16 by a basic carboxypeptidase.

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页码：126 / 128

页数：3

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