ISOLATION OF 37 SINGLE-COPY DNA PROBES FROM HUMAN CHROMOSOME-6 AND PHYSICAL MAPPING OF 11 PROBES BY INSITU HYBRIDIZATION

被引:7
作者
GOLUBIC, M
MATTEI, MG
VANCONG, N
FIGUEROA, F
KLEIN, J
机构
[1] UNIV MIAMI,SCH MED,DEPT MICROBIOL & IMMUNOL,MIAMI,FL 33152
[2] MAX PLANCK INST BIOL,IMMUNOGENET ABT,W-7400 TUBINGEN,GERMANY
[3] HOP ENFANTS,INSERM,U242,F-13385 MARSEILLE 5,FRANCE
[4] INST GUSTAVE ROUSSY,CNRS,CYTOGENET GENET ONCOL LAB 1158,F-94805 VILLEJUIF,FRANCE
关键词
D O I
10.1016/0888-7543(91)90262-D
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fifty-five single-copy DNA probes were isolated from the library LL06NS01, which was constructed from a complete HindIII digest of a flow-sorted human chromosome 6. Because chromosomes from a human × Chinese hamster somatic cell hybrid were used as the starting material for the flow-sorting, the library could be expected to contain some contaminating Chinese hamster DNA as well as DNA from human chromosomes other than 6. Thirty-seven of the 55 probes, however, were shown to map to human chromosome 6 by Southern blot hybridization with DNA from a panel of somatic cell hybrids. Eleven of the probes were mapped further by in situ hybridization. Four probes were localized to the short arm of chromosome 6, six to the long arm, and one to the centromeric region. © 1991.
引用
收藏
页码:338 / 343
页数:6
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