CHARACTERIZATION OF THE RDC1 GENE WHICH ENCODES THE CANINE HOMOLOG OF A PROPOSED HUMAN VIP RECEPTOR - EXPRESSION DOES NOT CORRELATE WITH AN INCREASE IN VIP BINDING-SITES

被引：23

作者：

COOK, JS

WOLSING, DH

LAMEH, J

OLSON, CA

CORREA, PE

SADEE, W

BLUMENTHAL, EM

ROSENBAUM, JS

机构：

[1] PROCTER & GAMBLE CO,MIAMI VALLEY LABS,POB 398707,CINCINNATI,OH 45239

[2] UNIV CALIF SAN FRANCISCO,SCH PHARM,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143

[3] YALE UNIV,SCH MED,DEPT PHARMACOL,NEW HAVEN,CT 06510

来源：

FEBS LETTERS | 1992年 / 300卷 / 02期

关键词：

RDC1; VIP RECEPTOR; RECEPTOR EXPRESSION; RECEPTOR CLONING; G-PROTEIN;

D O I：

10.1016/0014-5793(92)80184-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a portion of the canine gene encoding the orphan receptor RDC1 [1]. The complete coding sequence is contained in a single exon, and an intron divides the 5' untranslated region of RDC1 mRNA. The RDC1 protein is 94% homologous to the gene product of GPRN1, which has been proposed to serve as a VIP receptor when expressed in CHO-K1 and COS-7 cells (Sreedharan, S.P. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 4986-4990). Northern analysis indicates that CHO-K1 cells endogenously express a 2.1 kb RDC1 mRNA. However, while CHO-K1 cells possess detectable low affinity [I-125]VIP binding sites. VIP binding is not altered in membranes of CHO-K1 cells expressing varying amounts of the RDC1 gene construct. Further, endogenous VIP binding is not increased by transient expression of RDC1 in COS-7 cells. Taken together, the data suggest that RDC1 is not a canine homolog of the proposed VIP receptor.

引用

页码：149 / 152

页数：4

共 20 条

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