GAMMA-IRRADIATION OR HYDROCORTISONE TREATMENT OF RATS INCREASES THE PROTEINASE ACTIVITY ASSOCIATED WITH HISTONES OF THYMUS NUCLEI

被引：15

作者：

KUTSYI, MP

GAZIEV, AI

机构：

[1] Theoretical/Exptl. Biophysics Inst., Russian Academy of Sciences, Moscow Region, 142292, Pushchino

来源：

RADIATION RESEARCH | 1994年 / 140卷 / 02期

关键词：

D O I：

10.2307/3578906

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An increase in the activity of histone-associated rat thymus nucleus proteinases specific for histones H2A, H2B and H1 was shown after gamma irradiation or hydrocortisone treatment of animals. Histone III-specific proteinase activity is dependent on DNA and increases in the presence of denatured DNA, whereas proteinases specific for core histones are inhibited in the presence of denatured DNA. The increase in the activity of histone-associated proteinases depends on the radiation dose and the time after irradiation or hydrocortisone injection. In the presence of dithiothreitol and sodium dodecyl sulfate, these proteinases dissociate from histones. It was found by gel electrophoresis that several proteinases of various molecular masses are closely associated with histones obtained from thymus nuclei of irradiated or hydrocortisone-treated rats.

引用

页码：221 / 229

页数：9

共 43 条

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[2]

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