INTERACTION OF BOVINE GALLBLADDER MUCIN AND CALCIUM-BINDING PROTEIN - EFFECTS ON CALCIUM-PHOSPHATE PRECIPITATION

Background & Aims: Gallstones consist of calcium salts and cholesterol crystals, arrayed on a matrix of gallbladder mucin (GEM), and regulatory proteins like calcium-binding protein (CBP). To determine if interactions between CBP and GEM follow a biomineralization scheme, their mutual binding and effects on CaHPO4 precipitation were studied. Methods: Binding of CBP to GEM was assessed by inhibition of the fluorescence of the complex of GEM with bis-1,8-anilinonaphthalene sulfonic acid (bis-ANS). The effects of the proteins on precipitation of CaHPO4 were assessed by nephelometry and gravimetry. Precipitates were analyzed for calcium, phosphate, and protein. Results: CBP and bis-ANS competitively displaced each other from 30 binding sites on mucin, with a 1:1 stoichiometry and similar affinity. The rate of precipitation of CaHPO4 was retarded by mucin and CBP. Precipitate mass was unaffected by GEM alone but decreased with the addition of CBP. Complexing CBP with GEM abolished or moderated this latter effect, altered precipitate morphology, and changed the stoichiometric ratios of Ca to PO4 in the precipitates from 1:1 to 3:2. Mucin and CBP were incorporated into the precipitates. Conclusions: These studies suggest that the formation of calcium-containing gallstones is a biomineralization process regulated by both GEM and CBP.