A DNA SIGNAL FROM THE THY-1 GENE DEFINES DENOVO METHYLATION PATTERNS IN EMBRYONIC STEM-CELLS

被引：68

作者：

SZYF, M

TANIGAWA, G

MCCARTHY, PL

机构：

[1] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED,DIV HEMATOL,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT GENET,BOSTON,MA 02115

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 08期

关键词：

D O I：

10.1128/MCB.10.8.4396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although DNA can be extensively methylated de novo when introduced into pluripotent cells, the CpG island in the Thy-1 gene does not become methylated either in the mouse embryo or in embryonic stem cells. A 214-base-pair region near the promoter of the Thy-1 gene protects itself as well as heterologous DNA sequences from de novo methylation. We propose that this nucleotide sequence is representative of a class of important signals that limits de novo methylation in the embryo and establishes the pattern of hypomethylated CpG dinucleotides found in somatic tissues.

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页码：4396 / 4400

页数：5

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