FUNCTIONAL AND SEROLOGICAL ANALYSIS OF TYPE-II IMMUNOGLOBULIN G-BINDING PROTEINS EXPRESSED BY PATHOGENIC GROUP-A STREPTOCOCCI

被引:33
作者
RAEDER, R
OTTEN, RA
CHAMBERLIN, L
BOYLE, MDP
机构
[1] MED COLL OHIO,DEPT MICROBIOL,TOLEDO,OH 43699
[2] OHIO DEPT HLTH,COLUMBUS,OH 43266
关键词
D O I
10.1128/JCM.30.12.3074-3081.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacterial immunoglobulin-binding proteins expressed on the surface of group A streptococci represent a heterogeneous family of functionally related proteins. In this report, we describe efficient methods for extracting immunoglobulin-binding proteins and classifying them functionally and antigenically. A common characteristic of immunoglobulin-binding proteins expressed by group A streptococci appears to be the absence of internal methionine residues in the binding protein. This has enabled development of a rapid, efficient, cyanogen bromide-based extraction procedure for solubilizing these molecules from intact bacteria. Studies carried out with a series of monospecific polyclonal antibodies prepared in chickens have identified two major antigenic classes of immunoglobulin-binding proteins. The methods described in this report facilitate a rapid functional and serological screening of immunoglobulin-binding proteins that should now enable detailed epidemiological studies of the importance of these molecules in group A streptococcal infections and their relationship to other surface proteins, in particular, the antiphagocytic M protein.
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页码:3074 / 3081
页数:8
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