DYNAMICS OF EXOCYTOSIS, ENDOCYTOSIS AND RECYCLING IN SINGLE PITUITARY GONADOTROPES

被引：19

作者：

MASUMOTO, N ^{[1
]}

TASAKA, K ^{[1
]}

MIZUKI, J ^{[1
]}

TAHARA, M ^{[1
]}

MIYAKE, A ^{[1
]}

TANIZAWA, O ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT OBSTET & GYNECOL,2-2 YAMADAOKA,SUITA,OSAKA 562,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.2462

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dynamic changes in exocytosis, endocytosis and recycling in single gonadotropes induced by gonadotropin-releasing hormone (GnRH) were visualized and estimated wtih an impermeable fluorescent membrane probe, 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatrien (TMA-DPH), using a digital imaging and confocal laser scanning microscope. 10-7 M GnRH induced exocytosis and endocytosis within 10 sec and 60 sec, respectively. Recycling of the plasma membrane started at 180-300 sec. Exocytosis and endocytosis in purified gonadotropes changed dose-dependently with 10-10-10-7 M GnRH. These results show that GnRH-induced exocytosis, endocytosis and recycling in gonadotropes maintain dynamic equivalence. The procedure we established will be very useful in studies of the function of secretory cells. © 1993 Academic Press, Inc.

引用

页码：207 / 213

页数：7

共 15 条

[1] LABELING OF THE INNER SIDE OF GRANULES MEMBRANE DURING EXOCYTOSIS - A METHOD TO DIFFERENTIATE SECRETION PROCESSES [J].

BRONNER, C ;

KUHRY, JG ;

LANDRY, Y .

AGENTS AND ACTIONS, 1986, 18 (1-2) :53-56

[2] A FLUORESCENT HYDROPHOBIC PROBE USED FOR MONITORING THE KINETICS OF EXOCYTOSIS PHENOMENA [J].

BRONNER, C ;

LANDRY, Y ;

FONTENEAU, P ;

KUHRY, JG .

BIOCHEMISTRY, 1986, 25 (08) :2149-2154

[3] TOPOGRAPHICAL LOCALIZATION OF RECEPTORS FOR LUTEINIZING-HORMONE-RELEASING HORMONE ON SURFACE OF DISSOCIATED PITUITARY CELLS [J].

HOPKINS, CR ;

GREGORY, H .

JOURNAL OF CELL BIOLOGY, 1977, 75 (02) :528-540

[4] THE PLASMA-MEMBRANE INTERNALIZATION AND RECYCLING IS ENHANCED IN MACROPHAGES UPON ACTIVATION WITH GAMMA-INTERFERON AND LIPOPOLYSACCHARIDE - A STUDY USING THE FLUORESCENT-PROBE TRIMETHYLAMINODIPHENYLHEXATRIENE [J].

ILLINGER, D ;

POINDRON, P ;

GLASSER, N ;

MODOLLEL, M ;

KUHRY, JG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1030 (01) :82-87

[5] INTERNALIZATION OF THE LIPOPHILIC FLUORESCENT-PROBE TRIMETHYLAMINO-DIPHENYLHEXATRIENE FOLLOWS THE ENDOCYTOSIS AND RECYCLING OF THE PLASMA-MEMBRANE IN CELLS [J].

ILLINGER, D ;

POINDRON, P ;

FONTENEAU, P ;

MODOLLEL, M ;

KUHRY, JG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1030 (01) :73-81

[6] CAPACITANCE MEASUREMENTS - AN ANALYSIS OF THE PHASE-DETECTOR TECHNIQUE USED TO STUDY EXOCYTOSIS AND ENDOCYTOSIS [J].

JOSHI, C ;

FERNANDEZ, JM .

BIOPHYSICAL JOURNAL, 1988, 53 (06) :885-892

[7] PLASMA-MEMBRANE FLUIDITY MEASUREMENTS ON WHOLE LIVING CELLS BY FLUORESCENCE ANISOTROPY OF "TRIMETHYLAMMONIUMDIPHENYLHEXATRIENE [J].

KUHRY, JG ;

DUPORTAIL, G ;

BRONNER, C ;

LAUSTRIAT, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 845 (01) :60-67

[8] PATCH-CLAMP TECHNIQUES FOR TIME-RESOLVED CAPACITANCE MEASUREMENTS IN SINGLE CELLS [J].

LINDAU, M ;

NEHER, E .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1988, 411 (02) :137-146

[9]

MASUMOTO N, 1991, J BIOL CHEM, V266, P6485

[10] BINDING AND INTERNALIZATION OF NATIVE GONADOLIBERIN (GNRH) BY ANTERIOR-PITUITARY GONADOTROPHS OF THE RAT - A QUANTITATIVE AUTORADIOGRAPHIC STUDY AFTER CRYOULTRAMICROTOMY [J].

MOREL, G ;

DIHL, F ;

AUBERT, ML ;

DUBOIS, PM .

CELL AND TISSUE RESEARCH, 1987, 248 (03) :541-550

← 1 2 →