OVINE PLACENTAL-LACTOGEN LACKS DIRECT SOMATOGENIC AND ANTICATABOLIC ACTIONS IN THE POSTNATAL LAMB

被引:14
作者
OGAWA, E [1 ]
BREIER, BH [1 ]
BAUER, MK [1 ]
GLUCKMAN, PD [1 ]
机构
[1] UNIV AUCKLAND,SCH MED,DEV MED & BIOL RES CTR,DEPT PAEDIAT,AUCKLAND,NEW ZEALAND
关键词
D O I
10.1677/joe.0.1450087
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The metabolic effects of ovine placental lactogen (oPL) alone and in combination with bovine GH (bGH) were investigated in comparison with the identical dose of bGH alone in the well-fed postnatal lamb. The animals were treated by twice daily intramuscular injection for 5 days with oPL (n=7), bGH (n=7) or bGH+oPL (n=7) at a dose of 0.3 mg/kg per day or saline (n=9). bGH and bGH+oPL treatments, but not oPL treatment, resulted in significantly (P<0.01) higher plasma IGF-I levels than saline treatment. The rate of net protein catabolism (NPC) was significantly (P<0.05) reduced to the same extent by bGH and bGH+oPL treatment compared with saline treatment. In contrast, oPL did not affect the rate of NPC. Blood glucose and insulin:glucose ratio were significantly (P<0.05) elevated in the bGH+oPL group, whereas they were not significantly altered by either bGH or oPL treatment. These results suggest that oPL alone is not somatogenic or anticatabolic in the postnatal lamb despite the earlier evidence that oPL can bind to the oGH receptor (oGHR). However, oPL appeared to augment some of the effects of bGH when administered together, particularly with respect to carbohydrate metabolism. Potentiation of the diabetogenic effect of bGH by oPL may lead to insulin resistance during pregnancy. The lack of any obvious actions by oPL treatment alone may support the hypothesis that oGHR homo-dimerization is required for the full activation of GHR-mediated effects, since oPL binding does not initiate homo-dimerization of the oGHR.
引用
收藏
页码:87 / 95
页数:9
相关论文
共 46 条
[1]   USE OF A 96-WELL MICROPLATE READER FOR MEASURING ROUTINE ENZYME-ACTIVITIES [J].
ASHOUR, MBA ;
GEE, SJ ;
HAMMOCK, BD .
ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) :353-360
[2]   EFFECT OF BOVINE SOMATOTROPIN ON METABOLISM OF LACTATING DAIRY-COWS - INFLUENCE ON RATES OF IRREVERSIBLE LOSS AND OXIDATION OF GLUCOSE AND NONESTERIFIED FATTY-ACIDS [J].
BAUMAN, DE ;
PEEL, CJ ;
STEINHOUR, WD ;
REYNOLDS, PJ ;
TYRRELL, HF ;
BROWN, ACG ;
HAALAND, GL .
JOURNAL OF NUTRITION, 1988, 118 (08) :1031-1040
[3]   THE INDUCTION OF HEPATIC SOMATOTROPIC RECEPTORS AFTER BIRTH IN SHEEP IS DEPENDENT ON PARTURITION-ASSOCIATED MECHANISMS [J].
BREIER, BH ;
AMBLER, GR ;
SAUERWEIN, H ;
SURUS, A ;
GLUCKMAN, PD .
JOURNAL OF ENDOCRINOLOGY, 1994, 141 (01) :101-108
[4]   THE SOMATOTROPIC AXIS IN YOUNG STEERS - INFLUENCE OF NUTRITIONAL-STATUS ON PULSATILE RELEASE OF GROWTH-HORMONE AND CIRCULATING CONCENTRATIONS OF INSULIN-LIKE GROWTH FACTOR-I [J].
BREIER, BH ;
BASS, JJ ;
BUTLER, JH ;
GLUCKMAN, PD .
JOURNAL OF ENDOCRINOLOGY, 1986, 111 (02) :209-215
[5]   RADIOIMMUNOASSAY FOR INSULIN-LIKE GROWTH FACTOR-I - SOLUTIONS TO SOME POTENTIAL PROBLEMS AND PITFALLS [J].
BREIER, BH ;
GALLAHER, BW ;
GLUCKMAN, PD .
JOURNAL OF ENDOCRINOLOGY, 1991, 128 (03) :347-357
[6]   CHARACTERIZATION OF OVINE GROWTH-HORMONE (OGH) AND OVINE PLACENTAL-LACTOGEN (OPL) BINDING TO FETAL AND ADULT HEPATIC TISSUE IN SHEEP - EVIDENCE THAT OGH AND OPL INTERACT WITH A COMMON RECEPTOR [J].
BREIER, BH ;
FUNK, B ;
SURUS, A ;
AMBLER, GR ;
WELLS, CA ;
WATERS, MJ ;
GLUCKMAN, PD .
ENDOCRINOLOGY, 1994, 135 (03) :919-928
[7]  
BREIER BH, 1992, J ENDOCRINOLOGICA S4, V15, P55
[8]   RUMINANT PLACENTAL LACTOGENS - STRUCTURE AND BIOLOGY [J].
BYATT, JC ;
WARREN, WC ;
EPPARD, PJ ;
STATEN, NR ;
KRIVI, GG ;
COLLIER, RJ .
JOURNAL OF ANIMAL SCIENCE, 1992, 70 (09) :2911-2923
[9]   GROWTH-HORMONE ADMINISTRATION CONSERVES LEAN BODY-MASS DURING DIETARY RESTRICTION IN OBESE SUBJECTS [J].
CLEMMONS, DR ;
SNYDER, DK ;
WILLIAMS, R ;
UNDERWOOD, LE .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1987, 64 (05) :878-883
[10]   CLONING AND EXPRESSION OF OVINE PLACENTAL-LACTOGEN [J].
COLOSI, P ;
THORDARSON, G ;
HELLMISS, R ;
SINGH, K ;
FORSYTH, IA ;
GLUCKMAN, P ;
WOOD, WI .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (09) :1462-1469