PURIFICATION AND PROPERTIES OF DIMETHYL-SULFOXIDE REDUCTASE FROM RHODOBACTER-CAPSULATUS - A PERIPLASMIC MOLYBDOENZYME

被引：92

作者：

MCEWAN, AG

FERGUSON, SJ

JACKSON, JB

机构：

[1] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

[2] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1991年 / 274卷

关键词：

D O I：

10.1042/bj2740305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dimethyl sulphoxide reductase was purified from the photosynthetic bacterium Rhodobacter capsulatus. The enzyme is composed of a single polypeptide of M(r) 82000 and contains a pterin-type molybdenum cofactor as the only detectable prosthetic group. The oxidized molybdenum cofactor of dimethyl sulphoxide reductase is a weak chromophore and exhibits broad absorption bands in the u.v.-visible-absorption spectral region. A distinct spectrum was generated upon addition of dithionite.

引用

收藏

页码：305 / 307

页数：3

相关论文

共 16 条

[1] BACTERIAL REDUCTION OF TRIMETHYLAMINE OXIDE [J].

BARRETT, EL ;

KWAN, HS .

ANNUAL REVIEW OF MICROBIOLOGY, 1985, 39 :131-149

[2]

BASTIAN N R, 1988, Journal of Cell Biology, V107, p845A

[3] A MODEL FOR THE ACTIVE-SITES OF OXO-TRANSFER MOLYBDOENZYMES - REACTIVITY, KINETICS, AND CATALYSIS [J].

BERG, JM ;

HOLM, RH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (04) :925-932

[4] MOLYBDOPTERIN GUANINE DINUCLEOTIDE - A MODIFIED FORM OF MOLYBDOPTERIN IDENTIFIED IN THE MOLYBDENUM COFACTOR OF DIMETHYL-SULFOXIDE REDUCTASE FROM RHODOBACTER-SPHAEROIDES FORMA-SPECIALIS-DENITRIFICANS [J].

JOHNSON, JL ;

BASTIAN, NR ;

RAJAGOPALAN, KV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :3190-3194

[5] STRUCTURAL AND METABOLIC RELATIONSHIP BETWEEN THE MOLYBDENUM COFACTOR AND UROTHIONE [J].

JOHNSON, JL ;

RAJAGOPALAN, KV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (22) :6856-6860

[6]

JOHNSON JL, 1977, J BIOL CHEM, V252, P2017

[7] TRANSPOSON TN5 MUTAGENESIS OF GENES FOR THE PHOTOSYNTHETIC APPARATUS IN RHODOPSEUDOMONAS-CAPSULATA [J].

KAUFMANN, N ;

HUDIG, H ;

DREWS, G .

MOLECULAR & GENERAL GENETICS, 1984, 198 (01) :153-158

[8] ISOLATION OF TRANSPOSON TN5 INSERTION MUTANTS OF RHODOBACTER-CAPSULATUS UNABLE TO REDUCE TRIMETHYLAMINE-N-OXIDE AND DIMETHYLSULFOXIDE [J].

KELLY, DJ ;

RICHARDSON, DJ ;

FERGUSON, SJ ;

JACKSON, JB .

ARCHIVES OF MICROBIOLOGY, 1988, 150 (02) :138-144

[9] MODIFICATION OF LOWRY PROCEDURE TO SIMPLIFY PROTEIN DETERMINATION IN MEMBRANE AND LIPOPROTEIN SAMPLES [J].

MARKWELL, MAK ;

HAAS, SM ;

BIEBER, LL ;

TOLBERT, NE .

ANALYTICAL BIOCHEMISTRY, 1978, 87 (01) :206-210

[10] PERIPLASMIC LOCATION OF THE TERMINAL REDUCTASE IN TRIMETHYLAMINE N-OXIDE AND DIMETHYLSULFOXIDE RESPIRATION IN THE PHOTOSYNTHETIC BACTERIUM RHODOPSEUDOMONAS-CAPSULATA [J].

MCEWAN, AG ;

WETZSTEIN, HG ;

FERGUSON, SJ ;

JACKSON, JB .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 806 (03) :410-417