ADVENTITIOUS PESTIVIRUS RNA IN LIVE VIRUS-VACCINES AGAINST BOVINE AND SWINE DISEASES

被引：26

作者：

HARASAWA, R

机构：

[1] Animal Center for Biomedical Research, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo

来源：

VACCINE | 1995年 / 13卷 / 01期

关键词：

BOVINE VIRAL DIARRHEA VIRUS; CONTAMINATION; PESTIVIRUS;

D O I：

10.1016/0264-410X(95)80018-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Live virus vaccines against bovine and porcine diseases were examined for the presence of adventitious pestivirus RNA ol pestiviruses by reverse transcription-polymerase chain reaction (PCR). Pestivirus RNA was detected in the live virus vaccines against Akabane disease, Ibaraki disease, infectious bovine rhinotracheitis, porcine parvovirus infection, transmissible gastroenteritis and Japanese encephalitis. Pestivirus RNA or pestivirus in the fetal bovine serum wed to grow the host cells used to prepare the bovine and swine viral vaccines is a likely source of the contamination. Nucleotide sequence analysis of the PCR products suggests that modified live virus vaccines being used for immunization of cattle against bovine viral diarrhoea was not responsible for the contamination of the vaccines examined.

引用

页码：100 / 103

页数：4

共 35 条

[1] SURVEY OF CELL-LINES IN THE AMERICAN TYPE CULTURE COLLECTION FOR BOVINE VIRAL DIARRHEA VIRUS [J].