THE GENES ENCODING THE DELTA SUBUNITS OF DINITROGENASE-2 AND DINITROGENASE-3 ARE REQUIRED FOR MO-INDEPENDENT DIAZOTROPHIC GROWTH BY AZOTOBACTER-VINELANDII

被引:37
作者
WAUGH, SI
PAULSEN, DM
MYLONA, PV
MAYNARD, RH
PREMAKUMAR, R
BISHOP, PE
机构
[1] N CAROLINA STATE UNIV, USDA ARS, RALEIGH, NC 27695 USA
[2] N CAROLINA STATE UNIV, DEPT MICROBIOL, RALEIGH, NC 27695 USA
关键词
D O I
10.1128/jb.177.6.1505-1510.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
vnfG and anfG encode the delta subunits of alternative nitrogenases 2 and 3 in Azotobacter vinelandii, respectively. As a first step towards elucidating the role of these subunits, diazotrophic growth and acetylene reduction studies were conducted on mutants containing alterations in the genes encoding these subunits. Mutants containing a stop codon (C36stop) or an in-frame deletion in anfG were unable to grow in N-free, Mo-deficient medium (Anf(-)). Mutants in which cysteine 36 of AnfG (a residue conserved between VnfG and AnfG) was changed to Ala or Ser were Anf(+). Thus, this conserved cysteine is not essential for the function of AnfG in dinitrogenase 3. A mutant with a stop codon in vnfG (C17stop) grew after a lag of 25 h in N-free, Mo-deficient medium containing V2O5. However, a Nif(-) Anf(-) strain with this mutation was unable to grow under these conditions. This shows that the vnfG gene product is required for nitrogenase 2-dependent growth. Strains with mutations in vnfG and anfG reduced acetylene to different degrees. This indicates that the delta subunits are not required for acetylene reduction by nitrogenases 2 and 3.
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页码:1505 / 1510
页数:6
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