INDICATIONS OF ASSOCIATIVE NITROGEN-FIXATION IN EASTERN GAMAGRASS

被引:16
作者
BREJDA, JJ
KREMER, RJ
BROWN, JR
机构
[1] UNIV MISSOURI,SCH NAT RESOURCES,COLUMBIA,MO 65211
[2] UNIV MISSOURI,USDA ARS,CROPPING SYST & WATER QUAL UNIT,COLUMBIA,MO 65211
来源
JOURNAL OF RANGE MANAGEMENT | 1994年 / 47卷 / 03期
关键词
NONSYMBIOTIC NITROGEN-FIXATION; DIAZOTROPHIC BACTERIA; TRIPSACUM-DACTYLOIDES (L) L;
D O I
10.2307/4003014
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Associative nitrogen-fixation is one mechanism by which plants can meet a portion of their nitrogen (N) needs in N deficient environments. Eastern gamagrass [Tripsacum dactyloides (L.) L.] grows under conditions conducive for associative N-fixation. Our objective was to evaluate eastern gamagrass roots for nitrogenase activity and associated N-fixing bacteria, and compare its nitrogenase activity to switchgrass (Panicum virgatum L.). Soil and root samples were collected from unfertilized stands of ''PMK-24'' eastern gamagrass at 3 locations in northern Missouri and nitrogenase activity measured using the acetylene reduction method. Eastern gamagrass roots supported greater nitrogenase activity when colonized by indigenous bacteria, compared to roots inoculated with Klebsiella pneumoniae or surface sterilized. Eastern gamagrass roots colonized by indigenous bacteria had 17-46 fold greater nitrogenase activity than switchgrass roots. Bacterial composition on eastern gamagrass roots was dominated by fluorescent pseudomonads and Pseudomonas cepacia, with lower populations of other bacteria. Bacterial composition from incubation tubes showing nitrogenase activity was similar to that found on the roots, except that the diazotrophic bacterium Azospirillum braziliense was isolated from 1 of 3 sites. The potential for associative N-fixation with eastern gamagrass warrants further study and in situ measurement to quantify rates of N fixation.
引用
收藏
页码:192 / 195
页数:4
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