LOCALIZATION OF IMMUNOGLOBULIN LIGHT CHAIN MESSENGER-RNA EXPRESSION IN HODGKINS-DISEASE BY INSITU HYBRIDIZATION

被引：43

作者：

RUPRAI, AK

PRINGLE, JH

ANGEL, CA

KIND, CN

LAUDER, I

机构：

[1] UNIV LEICESTER,ROYAL INFIRM,DEPT PATHOL,LEICESTER LE2 7LX,W YORKSHIRE,ENGLAND

[2] UNIV SHEFFIELD,SCH MED,DEPT HISTOPATHOL,SHEFFIELD S10,ENGLAND

[3] FISONS PLC,DEPT PATHOL,DIV PHARMACEUT,LOUGHBOROUGH,LEICS,ENGLAND

来源：

JOURNAL OF PATHOLOGY | 1991年 / 164卷 / 01期

关键词：

HODGKINS DISEASE; INSITU HYBRIDIZATION; OLIGONUCLEOTIDE; MESSENGER RNA;

D O I：

10.1002/path.1711640107

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In situ hybridization techniques were used to detect immunoglobulin light chain messenger RNA (mRNA) in 28 formalin-fixed, paraffin-embedded samples of Hodgkin's disease. Cocktails of biotinylated oligonucleotide probes specific for the constant regions of kappa and lambda light chain mRNA were used. None of the Reed-Sternberg cells or their variants in any of the cases studied showed positive staining with either probe, in contrast to normal plasma cells which showed strong staining in the same sections. It was concluded, therefore, that the cytoplasmic immunoglobulin frequently detected within these cells by immunocytochemistry is present not as a result of synthesis, but as a result of some other mechanism.

引用

页码：37 / 40

页数：4

共 23 条

[1] INSITU HYBRIDIZATION DETECTION OF LIGHT CHAIN MESSENGER-RNA IN ROUTINE BONE-MARROW TREPHINES FROM PATIENTS WITH SUSPECTED MYELOMA [J].