TRANSCRIPTIONAL ATTENUATION CONTROL OF THE TYLOSIN-RESISTANCE GENE TLRA IN STREPTOMYCES-FRADIAE

被引：21

作者：

KELEMEN, GH

ZALACAIN, M

CULEBRAS, E

SENO, ET

CUNDLIFFE, E

机构：

[1] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,LEICS,ENGLAND

[2] LILLY CORP CTR,LILLY RES LABS,INDIANAPOLIS,IN 46285

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 14卷 / 04期

关键词：

D O I：

10.1111/j.1365-2958.1994.tb01319.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tylosin producer Streptomyces fradiae contains four known resistance genes, two of which (tlrA and tlrD) encode methyltransferases that act on ribosomal RNA at a common site. Expression of tlrA is regulated via transcriptional attenuation. A short transcript, only 411 nucleotides long, terminates 27 nucleotides into the methylase-coding sequence in the uninduced state. Induction of tlrA ii; proposed to involve a ribosome-mediated conformational change within the mRNA leader that allows transcription to continue beyond the attenuation sites, resulting in a transcript about 1450 nucleotides long. Transplantation of tlrD and/or tlrA into Streptomyces albus revealed that the induction specificity of tlrA depends upon the state of the ribosomes and is significantly altered in strains also expressing tlrD.

引用

页码：833 / 842

页数：10

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