ELIMINATION OF TWINNING IN CRYSTALS OF SULFOLOBUS-SOLFATARICUS ALCOHOL-DEHYDROGENASE HOLO-ENZYME BY GROWTH IN AGAROSE GELS

被引：19

作者：

SICA, F

DEMASI, D

MAZZARELLA, L

ZAGARI, A

CAPASSO, S

PEARL, LH

DAURIA, S

RAIA, CA

ROSSI, M

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,LONDON WC1E 6BT,ENGLAND

[2] CNR,IST BIOCHIM PROT & ENZIMOL,I-80125 NAPLES,ITALY

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1994年 / 50卷

关键词：

D O I：

10.1107/S0907444993013460

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of the binary complex of alcohol dehydrogenase from Sulfolobus solfataricus with NADH were shown to be twinned and not suitable for automated data collection. Several crystallization trials, performed with the aim of eliminating twinning, are described. Interestingly, crystals grown from agarose gel have been demonstrated to have a unique reciprocal lattice. These crystals are monoclinic, space group C2, with cell dimensions a = 134.47 (9), b = 85.26 (5), c = 71.76 (8) angstrom, beta = 97.53 (4)-degrees, and showed significant diffraction beyond 3.0 angstrom resolution.

引用

页码：508 / 511

页数：4

共 11 条

[1] THERMOSTABLE NAD+-DEPENDENT ALCOHOL-DEHYDROGENASE FROM SULFOLOBUS-SOLFATARICUS - GENE AND PROTEIN-SEQUENCE DETERMINATION AND RELATIONSHIP TO OTHER ALCOHOL DEHYDROGENASES [J].