IDENTIFICATION OF ALTERNATIVELY SPLICED VARIANTS OF TYPE-II PROCOLLAGEN IN VITREOUS

被引:59
作者
BISHOP, PN [1 ]
REARDON, AJ [1 ]
MCLEOD, D [1 ]
AYAD, S [1 ]
机构
[1] UNIV MANCHESTER,DEPT OPHTHALMOL,MANCHESTER M13 9PT,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1006/bbrc.1994.2180
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adult and foetal bovine vitreous were analyzed for the presence of unprocessed and partially processed forms of type II procollagen. Type II procollagen can exist in two alternatively spliced forms. a short form which lacks a large part of the N-propeptide due to the splicing out of exon 2 and a long form in which exon 2 is expressed and which has a full-sized N-propeptide. Both splice variants were demonstrated and analyses of type II pN-collagen demonstrated that the long form predominated, the ratio of the long form to the short form being 5:1 in foetal vitreous and 1.5:1 in adult vitreous. In foetal vitreous 35% of the type II collagen extracted was in the pN form, but there was very little unprocessed type II procollagen or type II pC-collagen. In contrast, adult vitreous extracts contained relatively less type II pN-collagen, but contained larger amounts of type II pC-collagen and unprocessed type II procollagen. (C) 1994 Academic Press, Inc.
引用
收藏
页码:289 / 295
页数:7
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