CONSTRUCTION INVITRO OF TRANSDUCING DERIVATIVES OF PHAGE-LAMBDA

被引：301

作者：

BORCK, K ^{[1
]}

BEGGS, JD ^{[1
]}

BRAMMAR, WJ ^{[1
]}

HOPKINS, AS ^{[1
]}

MURRAY, NE ^{[1
]}

机构：

[1] UNIV EDINBURGH, DEPT MOLEC BIOL, EDINBURGH EH9 3JR, SCOTLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1976年 / 146卷 / 02期

关键词：

D O I：

10.1007/BF00268089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods are described for the construction of plaque-forming, transducing derivatives of phage .lambda., using appropriate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the Escherichia coli trp, his, cysB, thyA, supD, supE, supF, hsd, tna and lig genes were isolated.

引用

页码：199 / 207

页数：9

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