MODIFIED CELLULAR-METABOLISM IN HYBRIDOMAS SUBJECTED TO HYDRODYNAMIC AND OTHER STRESSES

The responses of hybridoma growth and metabolism to hydrodynamic forces and changes in culture temperature have been examined. DNA synthesis is inhibited under conditions of intense hydrodynamic stress; however, cellular metabolic activity is increased perhaps to feed repair mechanisms. Sub-optimal temperature and nutrient deprivation increase cellular susceptibility to hydrodynamic stress, possibly through the inhibition of repair mechanisms. The reduced cell number in conditions of high agitation may be due at least in part to growth inhibition. © 1990 Kluwer Academic Publishers.