LOWRY PROTEIN ASSAY CONTAINING SODIUM DODECYL-SULFATE IN MICROTITER PLATES FOR PROTEIN DETERMINATIONS ON FRACTIONS FROM BRAIN-TISSUE

被引：75

作者：

HARRINGTON, CR

机构：

[1] Cambridge Brain Bank Laboratory, Department of Psychiatry, University of Cambridge School of Clinical Medicine, Cambridge, CB2 2QH, Hills Road

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 186卷 / 02期

关键词：

96-Well microtiter plate - Brain tissue - Microtiter plates - Modified lowry protein assays - Protein assays - Protein contents - Protein determination - Test samples;

D O I：

10.1016/0003-2697(90)90081-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modified Lowry protein assay which contains sodium dodecyl sulfate (Markwell et al., 1978, Anal. Biochem. 87, 207-210) has been adapted for use in 96-well microtiter plates. A spectrophotometer interfaced with a computer is used to plot the standard curve and calculate the protein content of each sample. The method is suitable for measuring 4-40 μg of protein/assay in a volume of 50 μl. There was no need for prior solubilization of proteins which had been extracted from brain tissue. Test samples, up to 21 in triplicate, can be tested on a single microtiter plate plus standards. Alternatively, further samples can be tested separately on extra plates using the standard curve from the original plate for calculations. © 1990.

引用

页码：285 / 287

页数：3

共 13 条

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