FREQUENCY OF MUTANT LYMPHOCYTES-T DEFECTIVE IN THE EXPRESSION OF THE T-CELL ANTIGEN RECEPTOR GENE AMONG RADIATION-EXPOSED PEOPLE

被引：51

作者：

KYOIZUMI, S

UMEKI, S

AKIYAMA, M

HIRAI, Y

KUSUNOKI, Y

NAKAMURA, N

ENDOH, K

KONISHI, J

SASAKI, MS

MORI, T

FUJITA, S

COLOGNE, JB

机构：

[1] RADIAT EFFECTS RES FDN,DEPT RADIOBIOL,5-2 HIJIYAMA PK,MINAMI KU,HIROSHIMA 732,JAPAN

[2] RADIAT EFFECTS RES FDN,DEPT STAT,HIROSHIMA 732,JAPAN

[3] KYOTO UNIV,SCH MED,DEPT NUCL MED,KYOTO 606,JAPAN

[4] KYOTO UNIV,CTR RADIAT BIOL,KYOTO 606,JAPAN

[5] NATL INST RADIOL SCI,DIV PHYSIOL & PATHOL,CHIBA 260,JAPAN

来源：

MUTATION RESEARCH | 1992年 / 265卷 / 02期

关键词：

SOMATIC MUTATION; T-CELL RECEPTOR GENE; RADIATION EXPOSURE;

D O I：

10.1016/0027-5107(92)90046-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The frequency of mutant T lymphocytes defective in T-cell receptor gene (alpha or beta) expression was measured using the 2-color flow cytometric technique. Results for a total of 203 atomic bomb survivors, 78 of whom were proximally exposed (DS86 doses of greater-than-or-equal-to 1.5 Gy) and 125 of whom were distally exposed (DS86 dose of < 0.005 Gy), showed that the mutant frequency was significantly higher in males than in females. No significant dose effects were observed. In contrast, a significant increase of mutant frequency was observed for 6 patients treated with Thorotrast, a contrast medium containing thorium-232 formerly used for radioligands. In addition, thyroid disease patients treated with I-131 showed a dose-related increase of mutant frequency. It was suggested that the present T-cell receptor mutation assay has a unique characteristic as a biological dosimeter for measurement of recent exposures to genotoxic agents.

引用

页码：173 / 180

页数：8

共 23 条

[1] MOLECULAR ANALYSIS OF SPONTANEOUS SOMATIC MUTANTS [J].

ADETUGBO, K ;

MILSTEIN, C ;

SECHER, DS .

NATURE, 1977, 265 (5592) :299-304

[2] PERIPHERAL LYMPHOCYTE-RESPONSE TO PHA AND T-CELL POPULATION AMONG ATOMIC-BOMB SURVIVORS [J].

AKIYAMA, M ;

YAMAKIDO, M ;

KOBUKE, K ;

DOCK, DS ;

HAMILTON, HB ;

AWA, AA ;

KATO, H .

RADIATION RESEARCH, 1983, 93 (03) :572-580

[3] AGE AND DOSE RELATED ALTERATION OF INVITRO MIXED LYMPHOCYTE CULTURE RESPONSE OF BLOOD-LYMPHOCYTES FROM A-BOMB SURVIVORS [J].

AKIYAMA, M ;

ZHOU, OL ;

KUSUNOKI, Y ;

KYOIZUMI, S ;

KOHNO, N ;

AKIBA, S ;

DELONGCHAMP, RR .

RADIATION RESEARCH, 1989, 117 (01) :26-34

[4] T-CELL CLONING TO DETECT THE MUTANT 6-THIOGUANINE-RESISTANT LYMPHOCYTES PRESENT IN HUMAN PERIPHERAL-BLOOD [J].

ALBERTINI, RJ ;

CASTLE, KL ;

BORCHERDING, WR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (21) :6617-6621

[5]

Anderson R E, 1976, Adv Immunol, V24, P215, DOI 10.1016/S0065-2776(08)60331-4

[6]

BROGGER A, 1982, SISTER CHROMATID EXC, P655

[7] THE T-CELL RECEPTOR/CD3 COMPLEX - A DYNAMIC PROTEIN ENSEMBLE [J].

CLEVERS, H ;

ALARCON, B ;

WILEMAN, T ;

TERHORST, C .

ANNUAL REVIEW OF IMMUNOLOGY, 1988, 6 :629-662

[8] INCREASED SOMATIC-CELL MUTANT FREQUENCY IN ATOMIC-BOMB SURVIVORS [J].

HAKODA, M ;

AKIYAMA, M ;

KYOIZUMI, S ;

AWA, AA ;

YAMAKIDO, M ;

OTAKE, M .

MUTATION RESEARCH, 1988, 201 (01) :39-48

[9] HIGH-RATES OF DELETIONS IN THE CONSTANT REGION SEGMENT OF THE IMMUNOGLOBULIN MU-GENE [J].

JACK, HM ;

WABL, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4934-4938

[10] MUTATIONS IN HUMAN-LYMPHOCYTES STUDIED BY AN HLA SELECTION SYSTEM [J].

JANATIPOUR, M ;

TRAINOR, KJ ;

KUTLACA, R ;

BENNETT, G ;

HAY, J ;

TURNER, DR ;

MORLEY, AA .

MUTATION RESEARCH, 1988, 198 (01) :221-226

← 1 2 3 →