SUBCELLULAR COMPARTMENTATION OF THE 4-AMINOBUTYRATE SHUNT IN PROTOPLASTS FROM DEVELOPING SOYBEAN COTYLEDONS

被引：62

作者：

BREITKREUZ, KE

SHELP, BJ

机构：

[1] UNIV GUELPH, DEPT HORT SCI, GUELPH, ON N1G 2W1, CANADA

[2] UNIV GUELPH, INTERDEPT PLANT PHYSIOL PROGRAM, GUELPH, ON N1G 2W1, CANADA

来源：

PLANT PHYSIOLOGY | 1995年 / 108卷 / 01期

关键词：

D O I：

10.1104/pp.108.1.99

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The subcellular localization of enzymes involved in the 4-aminobutyrate shunt was investigated in protoplasts prepared from developing soybean [Glycine max (L.) Merrill ev Maple Arrow] cotyledons. Protoplast lysate was fractionated by differential and continuous Percoll-gradient centrifugation to separate organelle fractions. Glutamate decarboxylase (EC 4.1.1.15) was found exclusively in the cytosol, whereas 4-aminobutyrate:pyruvate transaminase (EC 2.6.1.19) and succinic semialdehyde dehydrogenase (EC 1.2.1.16) were associated exclusively with the mitochondrial fractions. Mitochondrial fractions also catabolized [U-C-14]4-aminobutyrate to labeled succinate.

引用

页码：99 / 103

页数：5

共 27 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2] PHOTOSYNTHESIS AND CO2 EVOLUTION BY LEAF DISCS - GAS EXCHANGE, EXTRACTION, AND ION-EXCHANGE FRACTIONATION OF C-14-LABELED PHOTOSYNTHETIC PRODUCTS [J].