FREE-RADICAL FORMATION FROM ORGANIC HYDROPEROXIDES IN ISOLATED HUMAN POLYMORPHONUCLEAR NEUTROPHILS

被引：21

作者：

CHAMULITRAT, W

COHEN, MS

MASON, RP

机构：

[1] National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709

[2] Department of Medicine, Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1991年 / 11卷 / 05期

关键词：

ORGANIC HYDROPEROXIDES; MYELOPEROXIDASE; HUMAN POLYMORPHONUCLEAR NEUTROPHILS; FREE RADICALS; SPIN TRAPPING;

D O I：

10.1016/0891-5849(91)90059-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have demonstrated with electron paramagnetic resonance (EPR) that organic hydroperoxides are decomposed to free radicals by both human polymorphonuclear leukocytes (PMNs) and purified myeloperoxidase. When tert-butyl hydroperoxide was incubated with either PMNs or purified myeloperoxidase, peroxyl, alkoxyl, and alkyl radicals were trapped by the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO). In the case of ethyl hydroperoxide, DMPO radical adducts of peroxyl and alkyl (identified as alpha-hydroxyethyl when trapped by tert-nitrosobutane) radicals were detected. Radical adduct formation was inhibited when azide was added to the incubation mixture. Myeloperoxidase-deficient PMNs produced DMPO radical adduct intensities at only about 20-30% of that of normal PMNs. Our studies suggest that myeloperoxidase in PMNs is primarily responsible for the decomposition of organic hydroperoxides to free radicals. The finding of the free radical formation derived from organic hydroperoxides by PMNs may be related to the cytotoxicity of this class of compounds.

引用

页码：439 / 445

页数：7

共 36 条

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