HUMAN FIBROBLAST CELLS SYNTHESIZE AND SECRETE NERVE GROWTH-FACTOR IN CULTURE

被引：26

作者：

MURASE, K ^{[1
]}

MURAKAMI, Y ^{[1
]}

TAKAYANAGI, K ^{[1
]}

FURUKAWA, Y ^{[1
]}

HAYASHI, K ^{[1
]}

机构：

[1] GIFU PHARMACEUT UNIV,DEPT MOLEC BIOL,MITAHORA HIGASHI,GIFU 502,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91203-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using our enzyme immunoassay system developed for recombinant hNGF, we examined the synthesis and secretion of human NGF (hNGF) by human fibroblast (WS-1) cells. The amount of the factor secreted by WS-1 cells increased linearly and a significant amount of NGF was detected in the conditioned medium of WS-1 cultures. WS-1 NGF showed properties identical to those of recombinant human NGF in immunoreactivity and molecular weight. An increase in cell density or the withdrawal of serum from the culture medium caused a drastic decrease in the rate of NGF secretion. These results suggest that WS-1 cells are able to synthesize and secrete hNGF in culture and that the synthesis/secretion is regulated in a growth phase-dependent manner. © 1992.

引用

页码：373 / 379

页数：7

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