EFFECT OF GLUTATHIONE DEPLETION ON THE CONVERSION OF XANTHINE DEHYDROGENASE TO OXIDASE IN RAT-LIVER

被引：20

作者：

CIGHETTI, G ^{[1
]}

DEBIASI, S ^{[1
]}

PARONI, R ^{[1
]}

机构：

[1] SCI INST SAN RAFFAELE,MILAN,ITALY

来源：

BIOCHEMICAL PHARMACOLOGY | 1993年 / 45卷 / 11期

关键词：

D O I：

10.1016/0006-2952(93)90213-G

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The ability of endogenous glutathione (GSH) to modify the activity of the enzyme xanthine oxidase (XO) in rat liver was investigated. The effect of hepatic GSH depletion on the conversion of xanthine dehydrogenase (XDH) (EC 1.1.1.204) to XO (EC 1.1.3.22) was determined 10 min after i.p. administration of different amounts of diethylmaleate to fasted rats. After administration of 400 mg/kg, total hepatic non-protein GSH (reduced + oxidized GSH) decreased significantly to 14% of controls. In this condition the level of oxidized GSH was unchanged and no lipid peroxidation was observed, while a significant increase of reversible XO and a minor increase of the irreversible form of the enzyme was detected.

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页码：2359 / 2361

页数：3

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