COMPARISON OF PCR FINGERPRINTING, BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA, WITH OTHER MOLECULAR TYPING METHODS FOR CANDIDA-ALBICANS

被引：86

作者：

BOSTOCK, A ^{[1
]}

KHATTAK, MN ^{[1
]}

MATTHEWS, R ^{[1
]}

BURNIE, J ^{[1
]}

机构：

[1] UNIV MANCHESTER, DEPT MED MICROBIOL, STOPFORD BLDG, OXFORD RD, MANCHESTER M13 9PL, LANCS, ENGLAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-9-2179

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fingerprinting by random amplification of polymorphic DNA (RAPD) was compared with existing molecular typing systems for Candida albicans. Fifteen isolates were chosen, including three from the same patient; these gave 14 distinct karyotypes by pulsed-field gel electrophoresis (PFGE) and 7 different DNA types by EcoRI-generated restriction fragment length polymorphisms (RFLPs). RAPD with primer I (5'GCT GGT GG 3') gave 5 types, whereas primer II (5'GCG CAC GG3') yielded 11 types. Combining the results from both primers, all isolates were unique by RAPD with the exception of the three from the same patient. RAPD provided a fast, economical and reproducible means of typing C. albicans with a level of discrimination approaching that of PFGE.

引用

页码：2179 / 2184

页数：6

共 24 条

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