DEVELOPMENTAL REGULATION OF THE ALPHA-SUBUNIT OF G(Q)/G(11) AS DETERMINED BY QUANTITATIVE IMMUNOBLOT ANALYSIS

被引：8

作者：

MOCHIZUKI, N ^{[1
]}

URASAWA, K ^{[1
]}

SANCHEZ, A ^{[1
]}

INSEL, PA ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO, DEPT PHARMACOL, LA JOLLA, CA 92093 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1653

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We have used immunoblot analysis with glutathione and S-transferase fusion proteins as protein standards to examine expression of G(alpha q/II) in membrane preparations from different organs and to assess changes during normal development. Adult rat tissues expressed 4 -25 pmol/mg membrane protein G(alpha q/II) with neonates expressing two to three times higher levels in several tissues (brain, heart, liver, and kidney). By contrast, levels of G(aq/II) in lung were similar in adults and neonates. A G(aq)-specific antiserum confirmed the high expression in neonatal brain, heart, liver and kidney. Our results show that G(aq) is developmentally regulated in many rat tissues. (C) 1995 Academic Press, Inc.

引用

页码：246 / 252

页数：7

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